![]() ![]() At present, pomegranate is widely planted in subtropical and tropical regions, with a total planting area of more than 600 kha and an annual total output of about 6.0 million tons. It is native to Iran, Afghanistan and other Central Asian regions ( Verma et al., 2010). Pomegranate ( Punica granatum L.), one of the oldest fruits known to mankind, is a perennial deciduous shrub or arbor fruit tree ( Holland et al., 2009). This study provides a theoretical basis for understanding the evolution of higher plants, species classification and identification, and will also be useful for further utilization of pomegranate germplasm resources. The 580 and 432 RNA editing sites were predicted on 37 protein coding genes of mitochondrial genome using BEDTools software and online website PREPACT respectively, but all were from C to U, of which ccmB and nad4 gene were most frequently edited, with 47 sites. granatum had the closest genetic relationship with Lagerstroemia indica of Lythraceae. Phylogenetic analysis showed that among the published mitochondrial genomes of related genera, P. ![]() granatum mitochondrial genome, 14 homologous fragments of chloroplast genome were found, accounting for 0.54% of the total length. Besides, 400 pairs of dispersed repeats were detected, including 179 palindromic, 220 forward and one reverse. In the whole genome, 146 SSRs were identified. The total genome length was 404,807 bp, with the GC content of 46.09%, and there were 37 protein coding genes, 20 tRNA genes and three rRNA genes. granatum mitogenome had a multi branched structure, using BGI + Nanopore mixed assembly strategy. granatum was sequenced, assembled and analyzed in detail, while the chloroplast genome was assembled using the same set of data. In this study, the mitochondrial genome of P. However, there is no report on the mitochondrial genome of pomegranate. Pomegranate ( Punica granatum L.) is one of the oldest fruits with edible, medicinal and ornamental values. 2Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou, China.1Institute of Vegetables, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, China.Guilong Lu 1, Kai Zhang 1, Youxiong Que 2* and Yanfeng Li 1* ![]()
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